Lukkananukool, Achara

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Preferred name
Lukkananukool, Achara
Alternative Name
Lukkananukool, A.
Main Affiliation
Email
achara.lu@kmitl.ac.th

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    Chemical and fatty acid composition, collagen, calpain and troponin T contents and quality characteristics of five muscle types in native Thai cattle
    (2024-03-01)
    Chaosap, Chanporn
    ;
    Sommart, Kritapon
    ;
    Adeyemi, Kazeem D.
    ;
    Polyorach, Sineenart
    ;
    Lukkananukool, Achara
    In this study, chemical composition, fatty acids, collagen solubility, calpains, and troponin T degradation were investigated in various muscles of native Thai cattle (NTC). Five muscles, namely Bicep femoris, Infraspinatus, Longissimus thoracis, Supraspinatus and Semimembranosus were sampled from five Kho Isaan bulls. Moisture content of Longissimus thoracis was lower (p < 0.05) than others. Protein content of Longissimus thoracis, Bicep femoris and Semimembranosus was higher (p < 0.05) than that of Infraspinatus and Supraspinatus. Infraspinatus and Longissimus thoracis had higher fat content (p < 0.05) than Bicep femoris and Semimembranosus. Muscle pH, color, and drip loss did not differ between muscles. Semimembranosus and Supraspinatus showed higher cook loss (p < 0.05) than Infraspinatus and Longissimus thoracis. Infraspinatus had the lowest Warner–Bratzler shear force (p < 0.05). Supraspinatus, Infraspinatus and Bicep femoris had higher (p < 0.05) soluble, insoluble and total collagen content than Semimembranosus and Longissimus thoracis. Infraspinatus had higher collagen solubility than Semimembranosus and Supraspinatus. Collagen content and solubility were not affected by ageing time. The relative band intensities of intact troponin T and its degradation product at 2- and 14-days postmortem were higher in Longissimus thoracis than in other muscles. Infraspinatus and Supraspinatus had significantly higher expression of m-calpain and calpastatin genes than Longissimus thoracis, Bicep femoris, and Semimembranosus. Total fatty acid was higher while the percentage of C22:6n-3 was lower in Infraspinatus and Longissimus thoracis (p < 0.05) than in other muscles. These results show differences in specific nutritional, technological and eating quality characteristics of NTC muscles and thus provide information for optimizing storage and processing conditions for various NTC muscles.
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    Item type:Publication,
    Degradation of troponin-T associated with calpain/ calpastatin genes expression in native Thai beef cattle fed different levels of energy
    (2018-12-01)
    Nonsee, M.
    ;
    Lukkananukool, A.
    ;
    Polyorach, S.
    ;
    Sommart, K.
    ;
    Sazili, A. Q.
    The influence of dietary energy level on the degradation of troponin-T protein and expression of calpain and calpastatin genes in native Thai cattle was determined. Eighteen steers (at 200 days old and average body weight of 100 ±20 kg) were randomly assigned to 3 dietary treatment groups of different metabolizable energy: Treatment 1 [(8.9 MJ/ kg DM; n=6), Treatment 2 (9.7 MJ/kg DM; n=6) and Treatment 3 (10.5 MJ/kg DM; n=6)]. The diets were in the form of fermented total mixed ration (FTMR). At the end of 520 days of feeding, the steers attained 300 ± 10 kg BW at slaughter. The Longissimus dorsi m. samples collected within 2 d postmortem were further aged for 14 d for the troponin-T degradation immunoblot analysis while, the CAPN1, CAPN2, and CAST genes expression analyses only used samples at 2 d postmortem. The relative bands intensities of intact (37 kDa) and degraded troponin-T (30 kDa) were unaffected by the dietary treatments (p>0.05). Higher 37 kDa (p<0.01) and 30 kDa (p<0.001) troponin-T proteins expression were noted at 2 d and 14 d post-mortem, respectively. The CAPN1, CAPN2, and CAST genes expression remained unaffected (p>0.05) by the dietary treatment. Nonetheless, significant associations were observed between CAPN2 and CAST genes expression (r=0.93; p<0.0001), and between CAPN1 gene expression and 30 kDa troponin-T proteins expression at 14 d postmortem (r=0.58; p<0.05). This study concluded that neither troponin-T proteins nor calpain/ calpastatin genes expression were influenced by dietary energy levels in fattened native Thai cattle. The associations between CAPN2 and CAST genes expression, and between CAPN1 gene expression and 30 kDa troponin-T proteins expression at 14 d postmortem suggest the involvement of CAPN1 in myofibrillar proteolysis during the 14 d postmortem ageing of Longissimus dorsi m. in native Thai cattle.