Chutipaijit, Sutee

Preferred name

Chutipaijit, Sutee

Alternative Name

Chutipaijit, S.

Main Affiliation

Integrated Innovative Technology

sutee.ch@kmitl.ac.th

Now showing 1 - 10 of 21

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In vitro plant growth promotion by ZnO nanomaterials in indica rice seedlings (Oryza sativa L.)
(2018-01-01)
Chutipaijit, Sutee
;
Whalley, Anthony J.S.
;
Sutjaritvorakul, Thanawat
This research focuses on zinc oxide nanomaterials (nano-ZnO) applied to agricultural fields. Plants were exposed to nanomaterials and responded differently in tolerance to the various concentrations and types of nanomaterials. Two cultivars (SP1 and SP90) of indica rice (Oryza sativa L.) seedlings were grown at 0-1,000 mg.L-1 nano-ZnO and their morphological and physiological response was determined. Rice seedlings were harvested 5, 7, 10 and 14 days after treatments. Results demonstrated a significant increase in biomass, growth tolerance index (GTI), chlorophyll contents and antioxidant enzyme activities of the treated plant at 100 mg.L-1 nano-ZnO but these parameters decreased with increasing nano-ZnO concentration in the treated plants at 300-1,000 mg.L-1 nano-ZnO when compared to control plants (0 mg.L-1 nano-ZnO). The high accumulations of biomass, GTI and chlorophyll contents were related to production of antioxidant enzyme activities (catalase and peroxidase) in plant cells when plants were exposed to a low dose of nano-ZnO. Further investigations will determine the effect of nano-ZnO on the plant productivity.
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High contents of proline and anthocyanin increase protective response to salinity in Oryza sativa L. spp. indica
(2011-09-01)
Chutipaijit, Sutee
;
Cha-um, Suriyan
;
Sompornpailin, Kanokporn
Pigmented rice cultivar is a good candidate for abiotic tolerance especially salt tolerant trait. Physiological and growth parameters of eight indica rice genotypes under control or salt stress conditions were subjected to Hierarchical cluster analysis. From cluster ranking, rice genotypes were classified into two groups, salt-tolerant (Sangyod, Khao Dang, Kulab Dang and TD49) and salt-sensitive genotypes (Klum Sakol, Klum Khonkaen1, Klum Khonkaen2 and Black Sticky Rice). In salt stress (100 mM NaCl), the salt-tolerant genotypes showed the high percentages of proline and anthocyanin more than in the salt-sensitive genotypes. In addition, the photosynthetic pigment stabilizations of stressed-seedlings were positively correlated to the proline (R 2 = 0.98) and anthocyanin (R 2 = 0.70) accumulations. While, the malondialdehyde (MDA) contents of salt-stressed seedlings were negatively correlated to the proline (R 2 = 0.63) and anthocyanin (R 2 = 0.51) accumulations. Moreover, the total antioxidant activity of extraction from stressed-seedlings was assessed using the ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) method. The higher percentages of total antioxidant activity were determined in salt-tolerant (125-199%) than salt-sensitive genotypes (106-113%). From the results it can be concluded that accumulation of above mentioned metabolites is associated with cellular protection and salt detoxification in salinity rice seedlings.
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Establishment of condition and nanoparticle factors influencing plant regeneration from aromatic rice (Oryza sativa)
(2015-01-01)
Chutipaijit, Sutee
Improvements on the callus induction and regeneration stages as well as the applications of nanoparticles using aromatic rice cv. KDML105 were investigated in this study. An efficient induction of plant regeneration from mature seeds was found in calli cultured under dark condition and was not transferred to the fresh medium (non-subculture) in each culture stage. Although, the size and biomass factors showed the better performance in calli cultured under light condition than dark but the regeneration frequency rate was lower than dark condition. Between the types and concentrations of nanoparticles applied for the regeneration enhancement. ZnO nanoparticles were found to be toxic, while TiO2 nanoparticles improved the plant regeneration. Optimum concentration (25 mg L-1) of TiO<inf>2</inf> nanoparticles showed the high rate of green spots, plant regeneration and the ratio of the number of seedlings to the number of regenerated calli. Nonetheless, these factors may be valuable to establish an improvement in plant tissue culture system for propagation and/or genetic transformation in aromatic rice.
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Modifications of morphological and physiological characteristics of pigmented-rice seedlings by application of titanium dioxide nanoparticles
(2018-09-05)
Samart, Sutichai
;
Chutipaijit, Sutee
Nanomaterials or metal nanoparticles are considered as the agricultural applications with significant response-promoting influence. This research was focused on the influence of titanium dioxide nanoparticles (TiO<inf>2</inf> NPs) on morphological and physiological processes in pigmented-rice plants at seedling stage. A significant influence of TiO<inf>2</inf> NPs on the plant growth, photosynthetic pigment contents and antioxidant enzyme activities in rice seedlings was investigated. The results were also shown that pigmented-rice seedlings growing in TiO<inf>2</inf> NPs application significantly differed in the morphological and physiological responses when compared with the control plants. The results presented the plant height and dry weight was significantly affected by TiO<inf>2</inf> NPs. The results observed an increase in chlorophyll A, chlorophyll B, carotenoid contents and catalase, peroxidase enzyme activities in plant seedlings of TiO<inf>2</inf> NPs-treated plants which positive-corresponded with plant growth performances was found. TiO<inf>2</inf> NPs-treated seedlings that a high level of TiO<inf>2</inf> NPs application exhibited an increase in the plant growth, photosynthetic pigment contents and antioxidant enzyme activities. The amelioration effect of 800 mg L-1 of TiO<inf>2</inf> NPs application was shown the highest performances in morphological and physiological responses when compared with the other treatments. Investigation of the results suggests that the morphological and physiological responses due to changes in the plant growth, photosynthetic pigment content and antioxidant enzyme activity parameters may derive from the interaction between TiO<inf>2</inf> NPs and plant cells of pigmented-rice seedlings, which are known to play a role in plant development and productivity in the further study.
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Application of nanomaterials in plant regeneration of rice (Oryza sativa L.)
(2017-01-01)
Chutipaijit, Sutee
;
Sutjaritvorakul, Thanawat
In recent years, the application of nanomaterials has been investigated by various studies, especially in their biological and agricultural features. This research aimed to evaluate the influence of zinc oxide (ZnO) and titanium dioxide (TiO<inf>2</inf>) nanoparticles on the quality of the plant regeneration in rice (Oryza sativa L. cv. RD49). Mature seed explants were used for callus induction and plant regeneration cultured on the medium supplemented with different concentrations of ZnO or TiO<inf>2</inf> nanoparticles. The high percentage of the callus induction was achieved on the medium supplemented with 5 mg.L-1 ZnO nanoparticles or 20 mg.L-1 TiO<inf>2</inf> nanoparticles when compared with control treatment (without nanoparticles). Various concentrations of TiO<inf>2</inf> nanoparticles were also found to increase the percentage of the plant regeneration more than ZnO nanoparticles. The highest percentage of plant regeneration was observed on the medium containing the concentration of 20 mg.L-1 TiO<inf>2</inf> nanoparticles. The in vitro rooting of regenerated shoots was hardened and successfully transplanted to the field after 30 days of transplantation. The results suggested that ZnO and TiO<inf>2</inf> nanomaterials may have potential applications in the in vitro plant regeneration.
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Enhancement of plant regeneration efficiency from mature grains of thai indica rice (Oryza sativa L. cv. KDML105)
(2012-08-01)
Sompornpailin, Kanokporn
;
Chutipaijit, Sutee
In vitro protocol for efficient plant regeneration has been developed from Thai indica rice cultivar (Oryza sativa L. cv. KDML105). Embryogenic callus was initiated from mature seeds on NBMI medium. The highest frequency (96.00%) of callus induction was obtained on NBMI1 medium [2 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D) without kinetin]. These calli were sub-cultured on NBM medium supplemented with various concentrations of BA or combination with supporting materials for plant regeneration. Plant regeneration from embryogenic callus was increased in the medium supplemented with 5 g L-1 Phytagel® in the regeneration medium (NBMR9 medium; 30.00%). In addition, the best regenerated frequency in this experiment was obtained by culturing the embryogenic callus to 4:4 ratios of Gro-lux tubes to fluorescent light tubes at 1000 lux light intensity (56.72%). The regenerated plants were successfully transplanted to soil. The results indicated that manipulation of medium supplements and the cultured condition leads to increase plant regeneration efficiency in KDML105 cultivar for genetic transformation in the future.
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Proline accumulation and physiological responses of indica rice genotypes differing in tolerance to salt and drought stresses
(2010-06-01)
Chutipaijit, Sutee
;
Suriyan, Cha Um
;
Sompornpailin, Kanokporn
The study determined the relationship between proline accumulation and physiological responses of indica rice genotypes to salt and drought stresses in photoautotrophic conditions. Two salt-tolerant genotypes of indica rice [cv. jasmine rice (KDML 105) and Dang], two drought-tolerant genotypes [Rice Department 6 (RD6) and Rice Department 15 (RD15)] and a salt-and drought-sensitive genotype [Pathumthani 1 (PT 1)] were exposed to 100 mM NaCl (salt stress) or 100 mM mannitol (drought stress) for 4 d. The relative water content (RWC) and malondialdehyde (MDA) accumulation in salt-and drought-tolerant genotypes did not significantly change. The proline accumulation in these genotypes greatly increased with stress. On the other hand, the salt-and drought-sensitive genotype which had lower proline accumulation demonstrated a significant decrease in RWC and increase in MDA content. Moreover, our results showed a strong correlation between proline concentrations and free radical scavenging by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The high concentration of proline solution presented high DPPH antioxidant activity. The higher proline accumulation in stressed seedlings of tolerant genotypes may help to maintain the RWC and MDA of rice plant, which could lead to preferable growth performance.
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Titanium dioxide (TiO2) nanoparticles induced callus induction and plant regeneration of indica rice cultivars (suphanburi1 and suphanburi90)
(2018-10-01)
Chutipaijit, S.
;
Sutjaritvorakul, T.
Efficient plant regeneration systems are useful for indica rice using micropropagation and/or genetic transformation. Nanomaterials provide applications and opportunities in various fields of life science and agriculture technology. In this research, indica rice cultivars ‘Suphanburi1’ and ‘Suphanburi90’ (Oryza sativa L. ssp. indica) were used to investigate. Seeds were examined to evaluate callus induction and plant regeneration frequency. Culture of sterilized-seeds on callus induction medium supplemented with 50 mg L-1 of TiO<inf>2</inf> nanoparticles induced a high frequency of callus induction and content of biomass accumulation. Embryogenic calli were transferred to plant regeneration medium supplemented with 10-50 mg L-1 TiO<inf>2</inf> nanoparticles. The highest frequency of plant regeneration and the ratio of the number of seedlings to the number of regenerated calli were produced on plant regeneration medium supplemented with 40 mg L-1 of TiO<inf>2</inf> nanoparticles. The regenerated shoots induced roots on NB medium without plant growth regulators and the plantlets were established in soil. The results may be used for nanomaterial applications of rice and/or other plants for its further improvement.
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Growth of pigmented rice (Oryza sativa L. Cv. Riceberry) exposed to ZnO nanoparticles
(2019-01-01)
Samart, Sutichai
;
Chutipaijit, Sutee
Exposure of plants to nanomaterials has been evaluated to the positive and negative effects on crop growth, metabolism, and productivity. In this research, application of zinc oxide (ZnO) nanoparticles was studied in pigmented rice (Oryza sativa L. cv. Riceberry). Riceberry plants were grown and irrigated for 60 days. After that, Riceberry plants were exposed to ZnO nanoparticles by treating them with 0, 200, 400 or 800 mg L-1 of ZnO nanoparticles. The plant heights, plant weights, numbers of panicles/clump, photosynthetic pigment contents, and antioxidant enzyme activities were determined. Results showed that 200 mg L-1 of ZnO nanoparticles induced the high enhancement of plant heights, plant weights, numbers of panicles/clump, total photosynthetic pigment contents, and antioxidant enzyme activities as compared to 400 mg L-1, 800 mg L-1 of ZnO nanoparticles and the control (0 mg L-1 of ZnO nanoparticles) treatments. Moreover, the contents of anthocyanin accumulation in the rice seeds exposed to ZnO nanoparticles were measured. The seeds of Riceberry plants exposed to 200 mg L-1 of ZnO nanoparticles exhibited higher the accumulation of anthocyanin contents than other treatments (0, 400 and 800 mg L-1 of ZnO nanoparticles). It was observed that the appropriation of ZnO nanoparticle concentrations may play a role in enhanced the plant growth, productivity and metabolite of Riceberry plants.
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An evaluation of water deficit tolerance screening in pigmented indica rice genotypes
(2012-02-01)
Chutipaijit, Sutee
;
Cha-Um, Suriyan
;
Sompornpailin, Kanokporn
Eight pigmented genotypes of indica subspecies were geminated and then treated by mannitol-induced water deficit stress. A change of growth characteristics, photosynthetic pigments, lipid peroxidation, DNA content, proline content and anthocyanin accumulation in stressed seedling (100 mM mannitol) and control plant (0 mM mannitol) were calculated. Growth performances, photosynthetic pigment concentrations, and DNA contents in all rice genotypes were dropped whereas proline, anthocyanin contents and the lipid peroxidation levels were enriched. The stabilization in total photosynthetic pigment concentrations of stressed-seedlings were positively correlated to the proline or anthocyanin accumulation. In contrast, MDA content, the increases in the percentages of drought-stressed seedlings were negatively correlated to the proline or anthocyanin accumulation. The changes in biochemical, physiological and growth parameters were subjected to Ward's cluster analysis for water deficit tolerance. These cultivars could be classified into two groups, water deficit sensitive, SY, KD, KLD and TD49 and water deficit tolerance, KS, KK1, KK2 and BSR.

Chutipaijit, Sutee

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