Saetiew, Kanjana

Preferred name

Saetiew, Kanjana

Alternative Name

Saetiew, K.

Main Affiliation

School of Agricultural Technology

kanjana.sa@kmitl.ac.th

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Gene actions of resistance to anthracnose (Colletotrichum acutatum) of six F1 hybrid chili cultivars at the mature green fruit stage
(2023-01-01)
Sangarun, P.
;
Suwor, P.
;
Saetiew, K.
;
Tsai, W. S.
;
Techawongstien, S.
Anthracnose is an economically important fungal disease affecting the yield and fruit quality of chili in the tropics. This study aimed to assess the anthracnose disease reaction in six hybrid chili pepper cultivars compared with their parental lines. Two male resistant lines were crossed with three commercial female parents by North Carolina mating design II. All chili genotypes were grown in the greenhouse in a randomized complete block design with three replications, 10 plants each. Five mature green fruit plant-1 were harvested and inoculated with C. acutatum by using the microinjection method. ANT4 had smaller lesions than ANT10. Additionally, the F1-hybrids derived from ANT4 showed smaller disease lesions (7.47-9.82 mm) than on those derived from ANT10 (9.40-11.04 mm). However, the percentage heterosis of anthracnose disease traits in ‘Jindanil 80’ (JD) and ANT4 gave the lowest value to other parents. Hybrid JD × ANT4 showed the lowest anthracnose lesion size. However, the heterosis of cross the NM × ANT10 was the most negative at -43.68. The mode of gene action was found to be additive rather than involving dominant effects, with a narrow-sense heritability of 50.9% and broad sense heritability of 70.1%. Therefore, the resistant parent ANT4 cultivar is crucial for use as a resistant elite line for producing good F1 hybrid tolerance to anthracnose disease.
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Transformation of antisense dihydroflavonal 4-reductase (DFR) into sacred lotus 'Buntharik' using Agrobacterium-mediated gene transfer
(2014-03-13)
Saetiew, K.
;
Leethaweesup, W.
;
Parinthawong, N.
;
Arunyanart, S.
Transformation of antisense dihydroflavonal 4-reductase (DFR) into 'Buntharik' sacred lotus (Nelumbo nucifera Gaertn.) using Agrobacterium-mediated gene transfer was experimented. The A. tumefaciens strain EHA105 harbored two binary vectors. The pCAMBIA2301anti-DFR plasmid contained neomycin phosphotransferase (NPTII) as a selectable marker gene, the antisense DFR as an inserted gene and the β-glucuronidas gene (GUS) as a reportor gene. The pBI121anti-DFR plasmid contained NPTII gene and the antisense DFR. Twomonths-old calli regenerated from apical buds were used as plant materials. The plant materials were soaked in the Agrobacterium suspension for 10 or 30 min. The calli were co-cultivated for two days in the darkness. Treated calli were selected on solid MS medium containing 50 mg L-1 kanamycin and 250 mg L-1 cefotaxime. The calli were transferred to the same medium every two weeks for eight weeks. The survived calli regenerated shoots on MS medium containing a combination of 0.54 μM NAA and 4.44 μM BA. Both plasmids gave the best callus growth, maximum callus size and the survival percentage when soaked for 10 min. GUS bioassay was used to verify the presence of GUS gene in petioles and leaves of transgenic plants. It was found that petioles and leaves of transformants which were transformed with pCAMBIA2301anti-DFR by soaking for 30 min showed the highest percentage of blue spots on explants. Of all 14 PCR positive clones, 5 clones showed GUS gene, 11 clones contained NPTII gene and 6 clones had DFR gene.
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Peppermint essential oil extends the vase life of the cut Dendrobium orchid ‘White Sanan’
(2023-01-01)
Teerarak, M.
;
Saetiew, K.
;
Laosinwattana, C.
The vase life of cut orchid flowers is significantly extended by preservative solutions containing silver compounds. However, these compounds are toxic and require careful handling and disposal. As a result, the demand is increasing for natural preservation materials such as plant essential oils as they are biodegradable, ecofriendly, and safe alternatives for enhancing vase life and postharvest cut flower quality. The objective of this investigation was to study the effects of an emulsion of glucose and peppermint essential oil on vase life and postharvest quality of the popular cut Dendrobium orchid ‘White Sanan’. The orchids were preserved in vase solutions of distilled water or 4% glucose + 0.005-0.02% peppermint essential oil. The essential oil treatment increased the vase life of the flowers and the inflorescences by more than 6 days longer than the control treatment. The combination of glucose and peppermint essential oil increased water uptake and the fresh weight of cut orchids and delayed wilting time and abscission of floral buds and open florets. Moreover, treatment with the vase solution containing 4% glucose + 0.005% peppermint essential oil resulted in the highest bud opening rate (86.9%). Our results suggest that applying 4% glucose and 0.005-0.02% peppermint essential oil is especially effective as a preservative solution for the Dendrobium orchid ‘White Sanan’.
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Effects of organic compound and plant growth regulators on seed germination and growth of Doritis pulcherrima Lindl. in vitro
(2024-09-01)
Supreeworakij, S.
;
Saetiew, K.
;
Rungkitwattananukul, T.
;
Phanchompoo, P.
;
Montri, N.
Doritis pulcherrima Lindl. is one of the wild orchids of which the distribution has decreased in nature and endangered. In vitro seed culture by using plant tissue culture is a proper method to help increasing the numbers. The purpose of this research was to study the effect of organic compound and plant growth regulators on seed germination and growth of D. pulcherrima in vitro. Seeds were cultured on Vacin and Went (VW) basal medium. Experiments were performed in a 5×7 factorial in completely randomized design (factorial in CRD) 2 factors; Factor 1 capsule of D. pulcherrima with 5 treatments such as Pi102/1, Pi102/2, Pi119/1, Pi119/2 and Pu112. Factor 2 organic compound with 7 treatments such as no organic compound (control), 50, 100 g L-1 raw namwa banana powder, 50, 100 g L-1 cavendish banana homogenate, 50, 100 g L-1 potato homogenate. After 13 weeks of culture, there were only 3 capsules growth seed germination (Pi119/2, Pi102/2 and Pu112/1) and the highest percentage of seed germination was Pi119/2 on VW medium supplemented with 50, 100 g L-1 banana powder and 50 g L-1 potato homogenate gave the highest germination percentage at level 4 (75-100%). From the results, organic compound is a suitable culture supplement for D. pulcherrima seed germination and development compared to no organic compound. In addition, the effect of plant growth regulators on growth and development of D. pulcherrima protocorm were cultured on VW medium supplemented with different concentrations of control, 1, 2 and 3 mg L-1 BA and 0.01, 0.02 and 0.03 mg L-1TDZ. The result showed the control gave the highest number of leaves at 6.50, the number of protocorm at 3.67 and fresh weight of protocorm at 0.42 g. The protocorm and plantlet were induced after 10 of culture.
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Induction of calluses from petal of three chrysanthemum cultivars in vitro
(2024-09-01)
Boonprasop, S.
;
Saetiew, K.
;
Teeralak, M.
Propagation and breeding of chrysanthemum can be done via petal tissue culture technique. Since it has been well known that plant growth regulator has a rather wide range of physiological effects on diverse plant genetic resources, such effects were thus determined in petal induced callus of three chrysanthemum cultivars, i.e., ‘Pingpong Golden’, ‘Scarlet’ and ‘Karaked’. Chrysanthemum petals were detached from the plants of the three cultivars as mentioned then cultured on MS medium containing different formulas of plant growth regulators, i.e., 2 mg L-1 BA + 0.5 mg L-1 NAA; 2 mg L-1 BA + 1 mg L-1 2,4-D; 1 mg L-1 BA + 1 mg L-1 2,4-D + 1 mg L-1 Kinetin and 2 mg L-1 NAA + 4 mg L-1 Kinetin, as treatment 1-4, respectively. The cultures were put under white LED light (PPFD 40 µmol m-2 s-1) 16 h day-1 at 25±3°C for 12 weeks. The results showed that ‘Karaked’ exhibited the shortest callus induction period of 6-12 days and the highest fresh weight of callus growth (2.03-0.05 g) when cultured on MS medium supplemented with 1 mg L-1 BA + 1 mg L-1 2,4-D + 1 mg L-1 Kinetin. The callus ‘Scarlet’ performed highest chlorophyll content (chl a 98.59 mg g-1 FW, chl b 44.72 mg g-1 FW and carotenoid 24.26 mg g-1 FW) whereas compact callus with highest number of 4 shoots explant-1 were obtained in ‘Scarlet’ cultured on MS medium supplemented with 2 mg L-1 NAA + 4 mg L-1 Kinetin.
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Characterization of floral bud and anther development with stages of microspore in chili pepper (Capsicum annuum)
(2023-01-01)
Jaingulueam, T.
;
Suwor, P.
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Saetiew, K.
;
Tsai, W. S.
;
Techawongstien, S.
Anther culture can be used as a tool in breeding to shorten the time compared with the traditional method. Anther culture can produce double haploid plants quickly, hence saving cost and labor. The success of this technique depends on the use of microspores at optimum developmental stages. Therefore, the objective of this experiment was to determine the correlation between the day after flower formation (DAF), flower bud (FB) size, anther length, and microspore developmental stages in seven chili F1 hybrids. All parameters were observed at 0 to 10 DAF on three flowers per replication, three replications per cross. The study revealed a strong positive correlation between DAF and microspore developmental stages, with almost all crosses showing that 3 to 5 DAF correlated with the uninucleate stage. Moreover, a strong correlation was observed between petal/calyx ratio and the microspore developmental stages. However, we found overlapping stages of microspore in each stage and different cultivars showed a difference in FB size, anther length, and characteristics of anthers. Thus, for chili pepper, DAF could be used to effectively predict microspore development.
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Saetiew, Kanjana

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