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    Metal Oxide Nanostructures Enhanced Microfluidic Platform for Efficient and Sensitive Immunofluorescence Detection of Dengue Virus
    (2023-11-01)
    Pormrungruang, Pareesa
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    Phanthanawiboon, Supranee
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    Jessadaluk, Sukittaya
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    Larpthavee, Preeda
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    Thaosing, Jiraphon
    Rapid and sensitive detection of Dengue virus remains a critical challenge in global public health. This study presents the development and evaluation of a Zinc Oxide nanorod (ZnO NR)-surface-integrated microfluidic platform for the early detection of Dengue virus. Utilizing a seed-assisted hydrothermal synthesis method, high-purity ZnO NRs were synthesized, characterized by their hexagonal wurtzite structure and a high surface-to-volume ratio, offering abundant binding sites for bioconjugation. Further, a comparative analysis demonstrated that the ZnO NR substrate outperformed traditional bare glass substrates in functionalization efficiency with 4G2 monoclonal antibody (mAb). Subsequent optimization of the functionalization process identified 4% (3-Glycidyloxypropyl)trimethoxysilane (GPTMS) as the most effective surface modifier. The integration of this substrate within a herringbone-structured microfluidic platform resulted in a robust device for immunofluorescence detection of DENV-3. The limit of detection (LOD) for DENV-3 was observed to be as low as 3.1 × 10<sup>−4</sup> ng/mL, highlighting the remarkable sensitivity of the ZnO NR-integrated microfluidic device. This study emphasizes the potential of ZnO NRs and the developed microfluidic platform for the early detection of DENV-3, with possible expansion to other biological targets, hence paving the way for enhanced public health responses and improved disease management strategies.
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    The enhancement of sensitivity and response times of PDMS-based capacitive force sensor by means of active layer modification
    (2021-06-01)
    Siangkhio, Yasumin
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    ; ; ;
    Jessadaluk, Sukittiya
    In this work, sensitivity and response times of PDMS-based capacitive force sensors are enhanced via the modifications of the PDMS layer. Two modifying approaches are proposed; (i) change PDMS's (elastomer:curing agent) ratio and (ii) adding conductive polymer PEDOT:PSS into the PDMS layer. The change of PDMS (elastomer:curing agent) ratio from (10:1) to (30:1) increases the sensitivity from 0.4 0.08 to 0.72 0.23 kPa-1 (+80%) but it does not significantly affect the response/recovery times. In addition, by adding 1% wt. of PEDOT:PSS to PDMS (30:1), the further increment of sensitivity from 0.72 0.23 to 1.44 0.17 kPa-1 (+100%) and the shorter response time from 1.59 0.02 to 0.45 0.03 s (-72%) are observed. The mechanical and electrical studies reveal that the change of PDMS (elastomer:curing agent) ratio and the adding of PEDOT:PSS to PDMS layer result in the modification of PDMS's deformability and the increase of charge transportation, leading to the enhancement of sensing characteristics of the sensors.
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    Growth and characterization of novel optoelectronic materials. Based on II-VI inorganic/organic heterostructures
    (2006-01-01) ;
    Keawprajak, Anusit
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    ; ;
    Novel optoelectronic materials based on II-VI inorganic/organic low-dimensional heterostructure were successfully grown by electron beam evaporator. The structures were based on ZnSe, tris(8-hydroxyquinoline) aluminum (Alq<inf>3</inf>) and N,N'-bis(3-methylphenyl)-N,N'-diphenyl-benzidine (TPD). The surface morphology of the structures was investigated by atomic force microscopy and field emission scanning electron microscope. The optical and electronic properties were examined by photoluminescence, photocurrent and electroreflectance spectroscopy. Photoluminescence spectra of the ZnSe/Alq <inf>3</inf>/ZnSe structure attributed to the change of exciton energy as a result of quantum confinement showed the formation of single quantum well structure. The luminescence color can be varied by changing the thickness of the Alq<inf>3</inf> layer. The other heterostructure of ZnSe/Alq<inf>3</inf>/TPD was grown on silicon substrate. The wavelength response of this structure shown by photocurrent signal ranged from 450 nm to 1100 nm. Electroreflectance features due to optical transition energy of the single quantum well of this structure were also observed. Under applied voltage, electroreflectance signals showed significant shift due to the quantum confined Stark effect.
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    The decoration of ZnO nanoparticles by gamma aminobutyric acid, curcumin derivative and silver nanoparticles: Synthesis, characterization and antibacterial evaluation
    (2021-02-01)
    Talodthaisong, Chanon
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    Plaeyao, Kittiya
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    Mongseetong, Chatariga
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    Boonta, Wissuta
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    Srichaiyapol, Oranee
    Zinc oxide nanoparticles (ZnO NPs) are applied in various applications in catalysis, bio-sensing, imaging, and as antibacterial agents. Here we to prepare ZnO nanomaterials decorated by γ-amino butyric acid (GABA), curcumin derivatives (CurBF2) and silver nanoparticles (CurBF2-AgNPs). The structures of all ZnO nanostructures were characterized using Fourier transform in-frared (FT-IR) spectroscopy, X-ray diffraction (XRD), UV–VIS spectrophotometry, fluorescence spectrophotometry, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and high-resolution transmission electron microscopy (HR-TEM). Further, their antibacterial activities against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria were investigated through analysis of minimum inhibitory concentration (MIC) method. Among the prepared nanostructures, the ZnO NPs-GABA/CurBF2-AgNPs showed excellent antibacterial activity against both Gram-positive and-negative bacteria. ZnO NPs fabricated here may have potential use in future anti-bacterial compositions and coatings technologies.
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    Highly crystalline films of PCPDTBT with branched side chains by solvent vapor crystallization: Influence on opto-electronic properties
    (2015-02-18)
    Fischer, Florian S.U.
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    Trefz, Daniel
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    Back, Justus
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    Tornow, Benjamin
    (Figure Presented) PCPDTBT, a marginally crystallizable polymer, is crystallized into a new crystal structure using solvent-vapor annealing. Highly ordered areas with three different polymer-chain orientations are identified using TEM/ED, GIWAXS, and polarized Raman spectroscopy. The optical and structural properties differ significantly from films prepared by standard device preparation protocols. Bilayer solar cells, however, show similar performance.
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    Electroreflectance and photocurrent measurement of ZnSe/Alq 3/TPD heterostructure on Si-substrate
    (2005-11-20) ;
    Keawprajak, A.
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    ; ;
    The optical transition energy in ZnSe/tris(8-hydroxyquinoline) aluminum (Alq<inf>3</inf>)/N,N′-bis(3-methylphenyl)-N,N′-diphenyl-benzidine (TPD) heterostructure were investigated by room-temperature electroreflectance (ER) and photocurrent (PC) measurements. PC signal showed wavelength response of the device in the range of 450-1100 nm. ER features due to optical transition energy of the single quantum well of this structure were observed. The transition energies were determined by fitting the ER spectra to the theoretical line-shape expression. The subband transition energy decreased with increasing well thickness. Under applied voltage, both ER signals show significant shift due to the quantum confined Stark effect. © 2005 Elsevier B.V. All rights reserved.
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    Influence of Dragon Fruit Peels on the Synthesis of Antibacterial Nano Zinc Oxide (Nano-ZnO) via Green Synthesis Method
    (2026-05-20)
    Sakulpeeb, Natchayaporn
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    Koetniyom, Wantana
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    ; ;
    This research focused on adding value to dragon fruit peel waste by utilizing it in the synthesis of antibacterial nano zinc oxide (Nano-ZnO) through a green synthesis process. In this study, all the dragon fruit peels were extracted using the solvent extraction technique with three different solvents (deionized water, ethanol, and methanol) for 1, 2, 3, 4, and 5 h, respectively. The amount of flavonoids from the extract was determined using UV-Vis spectrophotometer to obtain the optimum extraction time, which was 4 h for DI water as the solvent. Moreover, antibacterial Nano-ZnO was synthesized successfully by a green synthesis process using zinc nitrate Zn(NO3)2 and the extracts. The molecular vibrations as well as the crystal structure and morphology were investigated by Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy (Raman), X-ray diffraction (XRD), and field emission scanning electron microscopy (FE-SEM), respectively. Additionally, the antibacterial efficacy of the nano-zinc oxide samples was evaluated using disc diffusion method. Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) were the test agents. The research shows that the X-ray diffraction patterns of all synthesized ZnO nanoparticles (NPs) exhibited a wurtzite (hexagonal) crystal structure. FT-IR spectroscopy confirmed the presence of Zn-O stretching vibrations at approximately 500 cm⁻¹. Furthermore, the FE-SEM reveals that ZnO-yellow particles displayed spherical morphologies with an average particle size of 145 nm. At the same time, ZnO-White and ZnO-Red nanoparticles exhibited a combination of rod-like and elliptical morphologies, with average particle sizes of 168 nm and 321 nm, respectively. In addition, the antibacterial activity demonstrates effective inhibition against S. aureus and E. coli in all three ZnO nanoparticle conditions.
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    A Shear-Thinning, Self-Healing, Dual-Cross Linked Hydrogel Based on Gelatin/Vanillin/Fe3+/AGP-AgNPs: Synthesis, Antibacterial, and Wound-Healing Assessment
    (2023-12-01)
    Talodthaisong, Chanon
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    Patramanon, Rina
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    Thammawithan, Saengrawee
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    Lapmanee, Sarawut
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    Maikaeo, Lamai
    A shear-thinning and self-healing hydrogel based on a gelatin biopolymer is synthesized using vanillin and Fe<sup>3+</sup> as dual crosslinking agents. Rheological studies indicate the formation of a strong gel found to be injectable and exhibit rapid self-healing (within 10 min). The hydrogels also exhibited a high degree of swelling, suggesting potential as wound dressings since the absorption of large amounts of wound exudate, and optimum moisture levels, lead to accelerated wound healing. Andrographolide, an anti-inflammatory natural product is used to fabricate silver nanoparticles, which are characterized and composited with the fabricated hydrogels to imbue them with anti-microbial activity. The nanoparticle/hydrogel composites exhibit activity against Escherichia coli, Staphylococcus aureus, and Burkholderia pseudomallei, the pathogen that causes melioidosis, a serious but neglected disease affecting southeast Asia and northern Australia. Finally, the nanoparticle/hydrogel composites are shown to enhance wound closure in animal models compared to the hydrogel alone, confirming that these hydrogel composites hold great potential in the biomedical field.
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    PMMA/High-k Self-assembled TiO2 /PMMA Multi-layer Gate Dielectric for P3HT Organic Field Effect Transistors
    (2022-01-01)
    Inpor, Kroekchai
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    Thanachayanont, Chanchana
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    Prichanont, Seeroong
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    In this work, a multi-layer structure of poly (methyl methacrylate)/ titanium dioxide/poly (methyl methacrylate) (PMMA/TiO₂ /PMMA; PTP) was proposed as a top-gate insulator for P3HT-based organic field-effect transistors (OFETs). Adding a TiO₂ interlayer as a high dielectric constant (high-k) material into PMMA film enables the modification of the dielectric constant of the multi-layers PTP film. The content of TiO₂ in the PTP film, which can be varied by changing the number of soaking cycles in TiO₂ solution, plays a crucial rule in modifying the dielectric constant of the PTP film. The higher the TiO₂ content used in the PTP film, the higher the dielectric constant of PTP film can be obtained. However, using high TiO₂ content led to a reduction in the dielectric constant of the PTP film due to leakage current induced by the agglomeration of TiO₂. The utilization of the top-gate insulator containing TiO₂ significantly enhanced several P3HT-OFETs characteristics, e.g., an increase in the I<inf>on</inf>/I<inf>off</inf> ratio, and a decrease in the threshold voltage. However, the use of the PTP top-gate insulator with a high content of TiO₂ resulted in regressions in the OFETs characteristics, such as a decrease in carrier mobility and reduction in the I<inf>on</inf>/I<inf>off</inf> ratio. OFETs operating at the optimum conditions of the PTP gate-insulator, with PTP thickness of 225 nm and RMS roughness of 20.0 nm, provided a dielectric constant of 7.13, a threshold voltage of-8.49 V, a saturation mobility of 2.2× 10<sup>-4</sup> cm²V<sup>-1</sup>s<sup>-1</sup>, I<inf>on</inf>/I<inf>off</inf> ratio of 37.9, and a subthreshold slope of 0.39 V/decade.
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    Simultaneous Improvement in Photovoltaic Performance and Air Stability of Perovskite Solar Cells by Controlling Molecular Orientation of Spiro-OMeTAD
    (2024-07-22)
    Sukgorn, Nuttaya
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    Kaewprajak, Anusit
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    Rodbuntum, Sasiphapa
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    Rujisamphan, Nopporn
    2,2′,7,7′-Tetrakis (N,N-di-p-methoxyphenyl-amine)9,9′-spirobifluorene (Spiro-OMeTAD) is a prototypical hole transport layer (HTL) for high-performance perovskite solar cells (PSCs). Since the electric conductivity of a neat Spiro-OMeTAD film is low, the HTL is generally doped with additives to increase charge density and mobility. However, the doped Spiro-OMeTAD film suffers from moisture absorption, which deteriorates the long-term stability of PSCs. This work reports that the molecular orientation of Spiro-OMeTAD molecules in the doped HTL is vital to solving this issue. Templating the molecular arrangement of Spiro-OMeTAD by a solidifying solvent, 1,3,5-trichlorobenzene (135-TCB), forms an anisotropic film of the doped Spiro-OMeTAD and induces a face-on orientation along the surface normal. Modifying the molecular orientation enhances hole mobility in the HTL and extraction of holes at the perovskite/HTL interface. As a result, the maximum power conversion efficiency (PCE) of the PSCs increases from 17.63 to 19.92%. Besides, the air stability of the PSCs with the face-on Spiro-OMeTAD, after storage for 1000 h, is superior to that of the devices without templating the molecular arrangement of Spiro-OMeTAD by 135-TCB. Control of the molecular orientation of Spiro-OMeTAD is critical for improving PCE and air stability.