Lukkananukool, Achara

Preferred name

Alternative Name

Lukkananukool, A.

Main Affiliation

School of Agricultural Technology

achara.lu@kmitl.ac.th

Now showing 1 - 6 of 6

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Evaluating the benefits of hemp leaves in goat diets: nutrient digestibility, growth performance and histomorphology
(2025-11-01)
Lukkananukool, Achara
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Kongrit, Chanya
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Chaosap, Chanporn
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Adeyemi, Kazeem D.
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Mitchaothai, Jamlong
This study investigated the influence of hemp leaves in concentrate diets on growth performance, nutrient digestibility, and intestinal morphology in goats. Fifteen Thai native × Boer bucks (BW: 16.28 ± 0.35 kg; 4 ± 0.5months old) were randomly assigned to diets containing 0, 2, or 4% hemp leaves and fed for 150 days. Results showed that feed intake, nutrient digestibility, and growth performance were unaffected by dietary hemp (P > 0.05), though goats fed 4% hemp leaves exhibited higher neutral detergent fiber intake. Blood urea nitrogen (BUN) levels were similar across all groups at 0 and 3 h postprandial, but goats fed 2% hemp leaves had elevated (P < 0.05) BUN at 6 h postprandial. Diets had no effect on the height, width, number and surface area of rumen papillae (P > 0.05). No significant differences were observed in villus height, villus width, or crypt depth in the duodenum, jejunum, and ileum. However, goats fed 4% hemp leaves displayed a significantly higher jejunal villus height to crypt depth ratio, indicating improved intestinal health. These findings suggest that hemp leaves can be included in goat diets without negative effects on performance, while the 4% enhances fiber intake and jejunal morphology.
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Chemical and fatty acid composition, collagen, calpain and troponin T contents and quality characteristics of five muscle types in native Thai cattle
(2024-03-01)
Chaosap, Chanporn
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Sommart, Kritapon
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Adeyemi, Kazeem D.
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Polyorach, Sineenart
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Lukkananukool, Achara
In this study, chemical composition, fatty acids, collagen solubility, calpains, and troponin T degradation were investigated in various muscles of native Thai cattle (NTC). Five muscles, namely Bicep femoris, Infraspinatus, Longissimus thoracis, Supraspinatus and Semimembranosus were sampled from five Kho Isaan bulls. Moisture content of Longissimus thoracis was lower (p < 0.05) than others. Protein content of Longissimus thoracis, Bicep femoris and Semimembranosus was higher (p < 0.05) than that of Infraspinatus and Supraspinatus. Infraspinatus and Longissimus thoracis had higher fat content (p < 0.05) than Bicep femoris and Semimembranosus. Muscle pH, color, and drip loss did not differ between muscles. Semimembranosus and Supraspinatus showed higher cook loss (p < 0.05) than Infraspinatus and Longissimus thoracis. Infraspinatus had the lowest Warner–Bratzler shear force (p < 0.05). Supraspinatus, Infraspinatus and Bicep femoris had higher (p < 0.05) soluble, insoluble and total collagen content than Semimembranosus and Longissimus thoracis. Infraspinatus had higher collagen solubility than Semimembranosus and Supraspinatus. Collagen content and solubility were not affected by ageing time. The relative band intensities of intact troponin T and its degradation product at 2- and 14-days postmortem were higher in Longissimus thoracis than in other muscles. Infraspinatus and Supraspinatus had significantly higher expression of m-calpain and calpastatin genes than Longissimus thoracis, Bicep femoris, and Semimembranosus. Total fatty acid was higher while the percentage of C22:6n-3 was lower in Infraspinatus and Longissimus thoracis (p < 0.05) than in other muscles. These results show differences in specific nutritional, technological and eating quality characteristics of NTC muscles and thus provide information for optimizing storage and processing conditions for various NTC muscles.
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Effects of supplementing hemp (Cannabis sativa L.) leaves in concentrate diet on carcass traits and meat characteristics of Longissimus muscle in goat kids
(2025-12-01)
Chaosap, Chanporn
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Sahatsanon, Katatikarn
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Adeyemi, Kazeem D.
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Sringarm, Korawan
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Mitchaothai, Jamlong
The influence of dietary hemp (Cannabis sativa L.) leaf (HL) supplementation in a concentrate diet on carcass traits, physicochemical properties, amino acids, ribonucleotides, lipid oxidation, and fatty acids of Longissimus thoracis et lumborum (LTL) muscle in goat kids was evaluated. Fifteen entire Thai native x Boer bucks (body weight: 16.28±0.35kg; age: 4 months) were randomly assigned to concentrate diets containing 0% (HL-0), 2% (HL-2), or 4% (HL-4) HL for 150 days, after which they were slaughtered. The LTL muscles were assessed for physicochemical properties, fatty acids, amino acids, ribonucleotides and lipid oxidation. The HL-4 goats had heavier hot and cold carcass weights compared to the other groups (P<0.05). Shoulder percentage was lower in the HL-0 goats than in the other groups (P<0.05). The diets did not affect color, chemical composition, shear force, muscle fiber diameter, sarcomere length, ribonucleotides, or free and total amino acids in the LTL muscle in goats. However, the HL-4 meat had higher (P<0.05) pH 24h compared to the HL-2 meat, while the pH of the HL-0 meat did not differ from that of the other groups. The HL-4 meat had lower cook loss compared to the other meats (P<0.05). HL supplementation increased C20:4n-6 concentration (P<0.05). HL-2 meat had higher total polyunsaturated fatty acids compared to the other groups (P<0.05). The HL-2 and HL-4 meats had lower TBARS values (P<0.05) throughout the 5-day postmortem chill storage. Dietary inclusion of 2–4% hemp leaves improved carcass yield, fatty acids, and oxidative stability of goat meat without compromising other quality traits, suggesting its potential as a natural feed additive to enhance meat quality and shelf life.
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Effects of Dietary Energy Density in a Fermented Total Mixed Ration Formulated with Different Ratios of Rice Straw and Cassava Pulp on 2- or 14-Day-Aged Meat Quality, Collagen, Fatty Acids, and Ribonucleotides of Native Thai Cattle Longissimus Muscle
(2022-07-01)
Chaosap, Chanporn
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Lukkananukool, Achara
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Polyorach, Sineenart
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Sommart, Kritapon
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Sivapirunthep, Panneepa
This study investigated the effects of dietary energy density in rice straw and cassava pulp fermented total mixed ration on pH, cooking loss, Warner–Bratzler shear force (WBSF), and collagen content of 2- or 14-d-aged native Thai cattle (NTC) Longissimus thoracic (LT) muscles and fatty acids and ribonucleotides of 2-d-aged LT. Eighteen yearling NTC (Bos indicus) were randomly divided into three dietary treatments (T1 = 8.9, T2 = 9.7, and T3 = 10.5 MJ ME/kg), with six bulls per treatment. The results showed that T1 had the highest WBSF (p < 0.05). However, T2 had similar WBSF to T3 (p > 0.05). With aging, cooking loss increased (p < 0.01), while WBSF decreased (p < 0.01). Insoluble and total collagen decreased with aging (p < 0.05). Dietary energy density had no effect (p > 0.05) on collagen content, ribonucleotides and most fatty acids. However, T1 had more (p < 0.05) decanoic (C10:0), vaccenic (C18:1n9t), trans-linolelaidic (C18:2n6t), eicosatrienoic (C20:3n6), and docosadienoic (C22:2) acids than T2 and T3. In terms of lowest feed cost with comparable tenderness to T2 and highest energy density, T3 may be well suited for feeding NTC. Aging for 14 days improves LT tenderness, but its cooking loss may affect yield and juiciness.
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Effect of Different Roughage Sources in Fermented Total Mixed Ration and Energy Intake on Meat Quality, Collagen Solubility, Troponin T Degradation, and Fatty Acids of Native Thai Cattle Longissimus Muscle
(2023-09-01)
Lukkananukool, Achara
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Polyorach, Sineenart
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Sommart, Kritapon
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Chaosap, Chanporn
The effects of roughage sources in the fermented total mixed ration (FTMR) and the level of energy intake on meat quality, collagen solubility, and troponin T degradation in longissimus thoracis (LT) muscle of native Thai cattle (NTC) were investigated. Results showed that roughage source affected fatty acid composition in the LT muscle (p < 0.05), as NTC fed Pakchong 1-Napier-based FTMR had higher monounsaturated fatty acid content and ω 6:ω 3 ratio. The high-energy ad libitum group had lower drip loss, lower shear force, and higher percent collagen solubility (p < 0.05). However, energy intake had no effect on troponin T degradation and fatty acid composition (p > 0.05). Longer aging of 14 days showed lower shear force values, higher collagen solubility, and troponin T degradation rate but higher cooking loss (p < 0.01). In conclusion, the meat quality of NTC could be improved by ad libitum feeding with NG-FTMR, as their meat had higher MUFA content, lower drip loss, lower shear force, and higher collagen solubility. In addition, the tenderness of NTC meat could be further improved by longer aging of 14 days post-mortem.
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Degradation of troponin-T associated with calpain/ calpastatin genes expression in native Thai beef cattle fed different levels of energy
(2018-12-01)
Nonsee, M.
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Lukkananukool, A.
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Polyorach, S.
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Sommart, K.
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Sazili, A. Q.
The influence of dietary energy level on the degradation of troponin-T protein and expression of calpain and calpastatin genes in native Thai cattle was determined. Eighteen steers (at 200 days old and average body weight of 100 ±20 kg) were randomly assigned to 3 dietary treatment groups of different metabolizable energy: Treatment 1 [(8.9 MJ/ kg DM; n=6), Treatment 2 (9.7 MJ/kg DM; n=6) and Treatment 3 (10.5 MJ/kg DM; n=6)]. The diets were in the form of fermented total mixed ration (FTMR). At the end of 520 days of feeding, the steers attained 300 ± 10 kg BW at slaughter. The Longissimus dorsi m. samples collected within 2 d postmortem were further aged for 14 d for the troponin-T degradation immunoblot analysis while, the CAPN1, CAPN2, and CAST genes expression analyses only used samples at 2 d postmortem. The relative bands intensities of intact (37 kDa) and degraded troponin-T (30 kDa) were unaffected by the dietary treatments (p>0.05). Higher 37 kDa (p<0.01) and 30 kDa (p<0.001) troponin-T proteins expression were noted at 2 d and 14 d post-mortem, respectively. The CAPN1, CAPN2, and CAST genes expression remained unaffected (p>0.05) by the dietary treatment. Nonetheless, significant associations were observed between CAPN2 and CAST genes expression (r=0.93; p<0.0001), and between CAPN1 gene expression and 30 kDa troponin-T proteins expression at 14 d postmortem (r=0.58; p<0.05). This study concluded that neither troponin-T proteins nor calpain/ calpastatin genes expression were influenced by dietary energy levels in fattened native Thai cattle. The associations between CAPN2 and CAST genes expression, and between CAPN1 gene expression and 30 kDa troponin-T proteins expression at 14 d postmortem suggest the involvement of CAPN1 in myofibrillar proteolysis during the 14 d postmortem ageing of Longissimus dorsi m. in native Thai cattle.

Lukkananukool, Achara

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