Chutipaijit, Sutee
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Preferred name
Chutipaijit, Sutee
Alternative Name
Chutipaijit, S.
Main Affiliation
Email
sutee.ch@kmitl.ac.th
31 results
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Item type:Publication, X-ray characterization, structural analysis, antibacterial activity, and self-cleaning property of Cu-doped TiO2-SiO2 nanocomposite prepared by sonochemical process(2024-12-01); ;Songpanit, Maneerat ;Sanyen, Thanyapa ;Samart, SutichaiTiO<inf>2</inf>-SiO<inf>2</inf> nanocomposites with different copper (Cu) precursor loadings were prepared by a one-step sonochemical process. The mole ratio of Cu precursor in TiO<inf>2</inf>-SiO<inf>2</inf> composite was varied at 0.004, 0.008, 0.020, and 0.040, respectively. The specific X-ray characterization techniques on crystalline structure, chemical composition, and chemical states of Cu-doped TiO<inf>2</inf>-SiO<inf>2</inf> composite were carried out by X-ray diffraction technique (XRD), X-ray fluorescence (XRF), and X-ray photoelectron spectroscopy (XPS), respectively. Surface morphology and chemical bonding of Cu-doped TiO<inf>2</inf>-SiO<inf>2</inf> composite were monitored by field emission scanning electron microscope (FE-SEM) and Fourier transform infrared spectrophotometer (FTIR). For antibacterial properties, the inhibition zone of antimicrobial activity was investigated by varying amounts of Cu precursors in the TiO<inf>2</inf>-SiO<inf>2</inf> composite. After that, Cu-doped TiO<inf>2</inf>-SiO<inf>2</inf> composite powder with different Cu precursor ratios was mixed in PMMA solution and deposited on glass slides to study the optical property and hydrophilicity by UV-VIS-NIR spectrophotometer and contact angle method. XRD patterns of Cu-doped TiO<inf>2</inf>-SiO<inf>2</inf> nanocomposites show the formation of the main TiO<inf>2</inf> anatase phase with the ultrafine particles observed by FE-SEM images. FT-IR spectra of the composites are assigned to the prominent peaks of the Ti-O-Ti and Ti-O-Si bond relating to the TiO<inf>2</inf>-SiO<inf>2</inf> host matrix. Meanwhile, TiO<inf>2</inf>-SiO<inf>2</inf> composites with Cu precursor at 0.004 mol ratio can significantly enhance the antibacterial activity with a large inhibition zone. The contact angle value of Cu-doped TiO<inf>2</inf>-SiO<inf>2</inf> nanocomposite film at 0.040 Cu precursor mole ratio in the TiO<inf>2</inf>-SiO<inf>2</inf> matrix resulted in the optimized composite ratio for achieving a hydrophilic surface on the substrate. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Green synthesis and optimized annealing tempserature effects on zinc oxide nanoparticles using mango peel extract(2026-01-01); ;Sakulpeeb, Natchayaporn ;Meedech, Woradech; Koetniyom, WantanaThis study presents the green synthesis of zinc oxide nanoparticles (ZnO-NPs) using mango peel extract (MgE) as a natural reducing and stabilizing agent. Phytochemical extraction was performed with deionized water, ethanol, and methanol for 1 h to 5 h, with deionized water at 4 h yielding the highest flavonoid content. Using zinc nitrate as a precursor, 25 mL of MgE successfully facilitated the synthesis of ZnO-NPs (ZnO-25MgE). It was found that annealing temperature at 400°C for 6 h produced pure ZnO. The obtained nanoparticles were characterized by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), and field emission scanning electron microscopy (FE-SEM). XRD confirmed the crystalline structure with an average size of 6.67 nm, while FT-IR and FE-SEM analyses revealed Zn–O vibrations, residual organics, and nanoscale morphology. Thermogravimetric analysis (TGA) indicated the appropriate annealing temperature for the removal of organics. For comparison, chemically synthesized ZnO was also characterized, and its antibacterial activity was assessed via disc diffusion against Escherichia coli and Staphylococcus aureus. The results demonstrated notable inhibition, highlighting the potential of biosynthesized ZnO-NPs for antimicrobial applications. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, High contents of proline and anthocyanin increase protective response to salinity in Oryza sativa L. spp. indica(2011-09-01); ;Cha-um, SuriyanPigmented rice cultivar is a good candidate for abiotic tolerance especially salt tolerant trait. Physiological and growth parameters of eight indica rice genotypes under control or salt stress conditions were subjected to Hierarchical cluster analysis. From cluster ranking, rice genotypes were classified into two groups, salt-tolerant (Sangyod, Khao Dang, Kulab Dang and TD49) and salt-sensitive genotypes (Klum Sakol, Klum Khonkaen1, Klum Khonkaen2 and Black Sticky Rice). In salt stress (100 mM NaCl), the salt-tolerant genotypes showed the high percentages of proline and anthocyanin more than in the salt-sensitive genotypes. In addition, the photosynthetic pigment stabilizations of stressed-seedlings were positively correlated to the proline (R <sup>2</sup> = 0.98) and anthocyanin (R <sup>2</sup> = 0.70) accumulations. While, the malondialdehyde (MDA) contents of salt-stressed seedlings were negatively correlated to the proline (R <sup>2</sup> = 0.63) and anthocyanin (R <sup>2</sup> = 0.51) accumulations. Moreover, the total antioxidant activity of extraction from stressed-seedlings was assessed using the ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) method. The higher percentages of total antioxidant activity were determined in salt-tolerant (125-199%) than salt-sensitive genotypes (106-113%). From the results it can be concluded that accumulation of above mentioned metabolites is associated with cellular protection and salt detoxification in salinity rice seedlings. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Biodiversity and bioactivity of stromatic xylariales collected from Mu Ko Chang National Park, Thailand(2020-01-01) ;Sutjaritvorakul, T.Ko Chang Island is a tropical rain forest island of Mu Ko Chang national park, Thailand, which has a wide variety of macro-fungi. The objective of this research was to study the biodiversity of Xylariales and to investigate the bioactive activity of ethyl acetate crude extracts. The fungi were collected from the forest area of the waterfalls all over the island. The crude extracts of selected fungi were tested for antibacterial activity by agar well diffusion method and the cancer cell viability was assessed using the MTT assay. Three genera of Xylariaceae and Hypoxylaceae were found including Xylaria, Daldinia and Hypoxylon. The crude extracts inhibited the growth of Gram positive bacteria more than that of Gram negative bacteria and showed broad spectrum inhibition to all cancer cell lines. The crude extract of Xylaria sp.3 showed the highest potential of antibacterial activity and selectively inhibited gastric carcinoma (Kato III) and T cell leukemia (Jurket) with the percentage survival of 26.12 ± 0.90% and 35.37 ± 0.40% respectively. The molecular identification of Xylaria sp.3 was identified as Xylaria sp.TP5BS101. These results show that these macromycetes are an excellent repertoire of antibacterial activity and exhibit anticancer activity against Kato III and Jurket by inducing DNA fragmentation. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Establishment of condition and nanoparticle factors influencing plant regeneration from aromatic rice (Oryza sativa)(2015-01-01)Improvements on the callus induction and regeneration stages as well as the applications of nanoparticles using aromatic rice cv. KDML105 were investigated in this study. An efficient induction of plant regeneration from mature seeds was found in calli cultured under dark condition and was not transferred to the fresh medium (non-subculture) in each culture stage. Although, the size and biomass factors showed the better performance in calli cultured under light condition than dark but the regeneration frequency rate was lower than dark condition. Between the types and concentrations of nanoparticles applied for the regeneration enhancement. ZnO nanoparticles were found to be toxic, while TiO2 nanoparticles improved the plant regeneration. Optimum concentration (25 mg L<sup>-1</sup>) of TiO<inf>2</inf> nanoparticles showed the high rate of green spots, plant regeneration and the ratio of the number of seedlings to the number of regenerated calli. Nonetheless, these factors may be valuable to establish an improvement in plant tissue culture system for propagation and/or genetic transformation in aromatic rice. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, The Effect of Polyvinyl Alcohol Addition on the Optical Properties and Oxygen Detection Performance of Titanium Dioxide and Methylene Blue Nanocomposite Colorimetric Indicators(2024-05-01); ;Rattan, Praphaporn ;Songpanit, Maneerat; Okumura, HideyukiIn this study, we investigated the impact of polyvinyl alcohol (PVA) incorporation on the optical properties and oxygen detection performance of a titanium dioxide/methylene blue (TiO<inf>2</inf>/MB) nanocomposite colorimetric indicator for packaging applications. The nanocomposite was synthesized via mechanical milling of TiO<inf>2</inf> nanoparticles with MB and citric acid. PVA, at varying concentrations (0, 3, 9, and 14 wt%), was introduced during the wet milling process to produce a homogeneous composite film. Spin coating was employed to fabricate TiO<inf>2</inf>/MB nanocomposite films for oxygen detection evaluation. The influence of PVA loading on the films’ chemical functionalities and surface morphologies was assessed using Fourier-transform infrared spectroscopy (FTIR) and field-emission scanning electron microscopy (FE-SEM). The indicator’s activation process, involving a color change between bleached and colored states, and its recovery time were monitored via optical imaging and UV-VIS-NIR spectrophotometry. The results revealed that a PVA content of 9 wt% yielded well-defined films with enhanced stability of the TiO<inf>2</inf>/MB nanocomposite’s oxygen detection performance. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Differential accumulations of proline and flavonoids in indica rice varieties against salinity(2009-10-01); ;Cha-Um, SuriyanFour varieties of indica rice differing in salt sensitivity were used for comparative study of defense systems in response to salinity. Rice seedlings were transferred into the photoautotrophic system containing NB medium with 100 mM NaCl as a salt stress treatment for 4 days. The physiological responses of jasmine rice (KDML105) and Sangyod (SY) varieties showed the better tolerance to salinity than those of Pathumthani 1 (PT1) and Black Sticky (BS) varieties. In salt stress treatment, the relative water content (RWC) of rice seedlings was decreased when compared to untreated seedlings. The RWC of salt-tolerant seedlings (KDML105 and SY) were decreased (0.33-0.53%) less than those of salt-sensitive seedlings (PT1 and BS), which exhibited 1.18-2.63% reductions. The salt-tolerant KDML105 and SY showed the lesser extent of membrane damage (lipid peroxidation; 7.94-19.26%) while enhanced the proline level (56.56-78.56%) and the flavonoid level (1.72-3.48%) more than those of the sensitive PT1 and BS varieties. Moreover, the positive correlation between proline or flavonoid accumulations and RWC was observed whereas the correlation between proline or flavonoid accumulations and MDA content was negative. These results suggest that high proline and flavonoids levels accumulated in each varieties of rice might be closely related to tolerant abilities indicating by physiological performances. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Influence of Dragon Fruit Peels on the Synthesis of Antibacterial Nano Zinc Oxide (Nano-ZnO) via Green Synthesis Method(2026-05-20) ;Sakulpeeb, Natchayaporn ;Koetniyom, Wantana; ; This research focused on adding value to dragon fruit peel waste by utilizing it in the synthesis of antibacterial nano zinc oxide (Nano-ZnO) through a green synthesis process. In this study, all the dragon fruit peels were extracted using the solvent extraction technique with three different solvents (deionized water, ethanol, and methanol) for 1, 2, 3, 4, and 5 h, respectively. The amount of flavonoids from the extract was determined using UV-Vis spectrophotometer to obtain the optimum extraction time, which was 4 h for DI water as the solvent. Moreover, antibacterial Nano-ZnO was synthesized successfully by a green synthesis process using zinc nitrate Zn(NO3)2 and the extracts. The molecular vibrations as well as the crystal structure and morphology were investigated by Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy (Raman), X-ray diffraction (XRD), and field emission scanning electron microscopy (FE-SEM), respectively. Additionally, the antibacterial efficacy of the nano-zinc oxide samples was evaluated using disc diffusion method. Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) were the test agents. The research shows that the X-ray diffraction patterns of all synthesized ZnO nanoparticles (NPs) exhibited a wurtzite (hexagonal) crystal structure. FT-IR spectroscopy confirmed the presence of Zn-O stretching vibrations at approximately 500 cm⁻¹. Furthermore, the FE-SEM reveals that ZnO-yellow particles displayed spherical morphologies with an average particle size of 145 nm. At the same time, ZnO-White and ZnO-Red nanoparticles exhibited a combination of rod-like and elliptical morphologies, with average particle sizes of 168 nm and 321 nm, respectively. In addition, the antibacterial activity demonstrates effective inhibition against S. aureus and E. coli in all three ZnO nanoparticle conditions. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Valorization of horse manure conversion to magnetic carbon nanofiber for dye adsorption by hydrothermal treatment coupled with carbonization(2024-06-01) ;Kaewtrakulchai, Napat ;Chanpee, Sirayu ;Pasee, Warit ;Putta, AmpolPollution of water resources has recently increased as a result of expanded industrial activity. Recycling waste biomass into bio-adsorbent material offers a cheap, easy, and eco-friendly solution. In this study, magnetic carbon nanofibers (MCNF) with a highly porous structure were developed from magnetite-preloaded horse manure by hydrothermal treatment followed by carbonization using different ratios of iron (III) nitrate and iron oxide as magnetic precursors. The produced MCNF had a very porous structure with specific surface area of 435.31 m<sup>2</sup>/g and high carbon content. The magnetic characteristics of MCNF promoted by the presence of iron oxide species. The saturated magnetization of MCNF obtained from a 5:5 ratio of the magnetic precursors (iron (III) nitrate: iron oxide) was 2.48 emu/g. Synthesized MCNF was applied as a bio-adsorbent for methylene blue (MB) removal from aqueous solution, with results showing excellent dye adsorption of 92–99 %. MB adsorption was facilitated by pore filling, electrostatic contact, hydrogen bonding, and ion complexation. Experimental results indicated that the Freundlich isotherm and pseudo-second-order kinetic models concurred with the observed MB adsorption data, suggesting that the adsorption mechanism involved multilayered micropore interactions between magnetite and MB chemisorption. The resulting magnetic adsorbent was successfully removed from the aqueous solution by physical separation. Findings indicated that horse manure-derived MCNF could be used as an efficient bio-adsorbent to remove organic contaminants in wastewater. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Enhancements of growth and metabolites of indica rice callus (Oryza sativa l. cv. pathumthani1) using tio2 nanoparticles (nano-tio2)(2020-04-01); Sutjaritvorakul, T.In this research, the effects of a various in concentration of TiO<inf>2</inf> nanoparticles (Nano-TiO<inf>2</inf>) were applied to study the cell growth, metabolites and antioxidant responses of callus in indica rice plants. Dose-dependent changes in growth performances, metabolite accumulations and antioxidant activities of callus were found. Mature seeds of indica rice cv. Pathumthani1 were cultured in callus induction medium with different concentrations of Nano-TiO<inf>2</inf> (0-600 mg L<sup>-1</sup>). After five weeks of cultivation, the callus formations were measured in fresh weights and dry weights, and the contents of phenolic compounds, flavonoids, and the antioxidant activities of callus extracts were assayed. The Nano-TiO<inf>2</inf> induced of fresh weights and dry weights in callus formations when compared with the control treatment (0 mg L<sup>-1</sup> Nano-TiO<inf>2</inf>) were also observed. The flavonoid and phenolic compound contents in rice callus exposed to 400 mg L<sup>-1</sup> Nano-TiO<inf>2</inf> were 118% and 139%, respectively, higher than rice callus unexposed to Nano-TiO<inf>2</inf>. Moreover, the antioxidant activities of the callus metabolites studied shows that the percentages of inhibition were up-regulated (84%) when exposed to 400 mg L<sup>-1</sup> Nano-TiO<inf>2</inf>, as a response to the high-level depletion of the free radicals in metabolites of rice callus. The results obtained from this research showed that the addition of Nano-TiO<inf>2</inf> had impacts on the callus growth and metabolite accumulations in rice callus. The results suggested that addition of Nano-TiO<inf>2</inf> at appropriate levels could role as an elicitor for biosynthesis of valuable metabolites and antioxidant properties for pharmaceutical applications in further research.
