Now showing 1 - 10 of 25
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    Item type:Publication,
    A Novel Approach for Optimizing Molecularly Imprinted Polymer Composition in Electrochemical Detection of Collagen Peptides
    (2025-11-01)
    Vongmanee, Naphatsawan
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    Rattanapithan, Katesirin
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    Sriwichai, Phuritasinee
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    Collagen peptides are key structural proteins that play an important role in maintaining the integrity and proper function of multiple tissues in the human body. Their breakdown is recognized as an important biomarker for various degenerative conditions, including the loss of muscle mass, joint and bone disorders, and compromised skin health. Current analytical approaches for collagen detection, such as ultraviolet spectrometry, enzyme-linked immunosorbent assay (ELISA), high-performance liquid chromatography (HPLC), and histochemical staining, are widely used but often expensive, time-consuming, and reliant on specific laboratory instrumentation, limiting their practicality for routine or rapid diagnostics. This study reports a novel biosensor for collagen peptide detection based on molecularly imprinted polymers (MIPs) integrated with screen-printed electrodes (SPEs). Electrochemical measurements revealed a clear correlation between collagen concentration and current response, confirming effective molecular binding within the imprinted matrix. The optimized MIP-modified electrode exhibited a detection range of 0.1–1000 µg/mL with a limit of detection (LOD) of 1.0106 µg/mL, limit of quantification (LOQ) of 4.46 µg/mL, sensitivity of 8.3816, and correlation coefficient (R<sup>2</sup> = 0.9436). These results highlight strong selectivity and sensitivity toward collagen peptides. The proposed MIP-based biosensor provides a rapid, low-cost platform for detecting collagen degradation products and holds potential for early diagnosis and future clinical applications in degenerative disease monitoring.
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    Smart wheelchair based on eye tracking
    (2017-02-21)
    Wanluk, Nutthanan
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    Juhong, Aniwat
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    This project is a smart wheelchair based on eye tracking which is designed for people with locomotor disabilities. The add-on controlled module can be used with any electrical wheelchair. The smart wheel chair consists of four modules including imaging processing module, wheelchair-controlled module, SMS manager module and appliance-controlled module. The image processing module comprises of a webcam installed on the eyeglass and C++ customized image processing software. The captured image which is transmitted to raspberry Pi microcontroller will be processed using OpenCV to derive the 2D direction of eye ball. The coordinate of eyeball movement is then wirelessly transmitted to wheelchair-controlled module to control the movement of wheel chair. The wheelchair-controlled module is two dimensional rotating stages that installed to the joystick of the electrical wheelchair to replace the manual control of the wheelchair. The motion of eyeball is also used as the cursor control on the raspberry Pi screen to control the operation of some equipped appliance and send message to smart phone.
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    Automated segmentation of infarct lesions in t1‐weighted mri scans using variational mode decomposition and deep learning
    Automated segmentation methods are critical for early detection, prompt actions, and immediate treatments in reducing disability and death risks of brain infarction. This paper aims to develop a fully automated method to segment the infarct lesions from T1‐weighted brain scans. As a key novelty, the proposed method combines variational mode decomposition and deep learning-based segmentation to take advantages of both methods and provide better results. There are three main technical contributions in this paper. First, variational mode decomposition is applied as a pre-processing to discriminate the infarct lesions from unwanted non‐infarct tissues. Second, overlapped patches strategy is proposed to reduce the workload of the deep‐learning‐based segmentation task. Finally, a three‐dimensional U‐Net model is developed to perform patch‐wise segmentation of infarct lesions. A total of 239 brain scans from a public dataset are utilized to develop and evaluate the proposed method. Empirical results reveal that the proposed automated segmentation can provide promising performances with an average dice similarity coefficient (DSC) of 0.6684, intersection over union (IoU) of 0.5022, and average symmetric surface distance (ASSD) of 0.3932, respectively.
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    Automatic detection of pulmonary nodules using three-dimensional chain coding and optimized random forest
    The detection of pulmonary nodules on computed tomography scans provides a clue for the early diagnosis of lung cancer. Manual detection mandates a heavy radiological workload as it identifies nodules slice-by-slice. This paper presents a fully automated nodule detection with three significant contributions. First, an automated seeded region growing is designed to segment the lung regions from the tomography scans. Second, a three-dimensional chain code algorithm is implemented to refine the border of the segmented lungs. Lastly, nodules inside the lungs are detected using an optimized random forest classifier. The experiments for our proposed detection are conducted using 888 scans from a public dataset, and achieves a favorable result of 93.11% accuracy, 94.86% sensitivity, and 91.37% specificity, with only 0.0863 false positives per exam.
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    Emergency Blower-Based Ventilator with Novel-Designed Ventilation Sensor and Actuator
    (2022-03-01) ;
    Maneerat, Areerat
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    The ventilator, a life-saving device for COVID-19-infected patients, especially for pneu-monia patients whose lungs are infected, has overwhelmingly skyrocketed since the pandemic of COVID-19 diseases started in December 2019. As a result, many biomedical engineers have rushed to design and construct emergency ventilators, using the Ambu-bag squeezing ventilator to compensate for the insufficient ventilators supply. The Ambu-bag squeezing ventilator, however, suffers from the limitation of delivered tidal volume to the patient, the setting respiration rate and the noisy operational sound due to the movement of mechanic parts. The Ambu-bag based ventilator is, hence, not suitable for prolonged treatment of the patient. This paper presents a design and construction of a blower-based pressure-controlled ventilator for home-treatment COVID-19 patients featured with our novel-designed flow and pressure sensor, electronic peep valve and proportional controlled valve. Our proposed ventilator can be programmed with the suitable parameter setting depending upon the weight, height, gender, and blood oxygen saturation (SpO<inf>2</inf>) of the patients. This is useful in the current situation of COVID-19 pandemics, where trained medical staff is limited. The designed ventilator is also equipped with a safety mechanism, including an excessive-pressure-release valve, excessive flow rate, overpressure, and over-temperature blower to prevent any hazardous event. A home ventilator server is also set where all ventilator parameters will be acquired and broadcasted for remote access of the health provider. The designed blower-based ventilator has been calibrated and evaluated with a lung simulator and standard ventilator tester, including alarmed functions, safety mechanism, sound level, and regulated pressure. The respiration output graph is complied with the simulation. The blower-based ventilator for home-treatment COVID-19 patients is suitable for life support, commensurate with the strict requirements of the FDA for life-support ventilators, and ready to be tested with animal subjects in the next phase.
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    Optical-based foot plantar pressure measurement with application in human postural balance, gait and recognition analysis
    In this research, we purposed the design of real-time low-cost marker-free optical-based plantar-pressure measurement with application in human postural control, gait and recognition analysis. The system consists of a series of digital cameras capture installed underneath the acrylic-top platform. Light of LED strip mounted along the side of the acrylic plate deflected by the pressure exerted between the glossy white paper installed in the top of acrylic and the acrylic plate to the digital cameras provides the color-coded plantar pressure image of the subject standing on the platform. The system can provide both static features and dynamic features. Our hybrid system consists of a series of USB cameras aligned under the acrylic-plate walking platform. The mosaic image processing is used to concatenate the captured image to increase the sensing area. Captured the image data in video mode, various dynamic parameters can be derived for further dynamic analysis. The image capturing in one specific frame can be used for static analysis. Application in human-postural measurement, gait analysis, and person identification indicate that our system is an all-in-one system for plantar pressure measurement.
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    Ultrasensitive Label-Free Electrochemical Detection of Pseudomonas aeruginosa Using a Surface Molecularly Imprinted Polymer-Modified Screen-Printed Electrode
    (2026-06-01)
    Vongmanee, Naphatsawan
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    Pseudomonas aeruginosa is a major opportunistic pathogen frequently associated with nosocomial infections, such as pneumonia, urinary tract infections, and wound infections, particularly in immunocompromised or hospitalized patients. These infections are often difficult to treat due to the pathogen’s intrinsic antibiotic resistance and biofilm-forming ability. Therefore, rapid and selective detection of P. aeruginosa is essential for early diagnosis and effective infection control. In this study, a novel surface-imprinted MIP design uniquely combines methacrylamide (MAM), acrylamide (AAM), and vinylpyrrolidone (VP) monomers to generate recognition cavities that are complementary to the surface morphology and physicochemical properties of Pseudomonas aeruginosa cells. Unlike traditional MIP approaches, this surface imprinting strategy provides improved stability and reproducibility, without relying on biological recognition elements like antibodies or aptamers. This novel approach enabled us to achieve an ultralow LOD of 1 CFU/mL over a linear range of 1–10<sup>4</sup> CFU/mL, demonstrating excellent analytical performance. In addition, the sensor exhibited good reproducibility with an RSD of 5–12%. The novelty of this work lies in the use of a surface-imprinted MIP strategy combined with a multi-monomer system to enhance bacterial recognition and sensing performance. Overall, the proposed MIP-based electrochemical biomimetic sensor offers a rapid, cost-effective, and portable platform with strong potential for the detection of P. aeruginosa in clinical and environmental applications.
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    Electrochemical Characterization of a Molecularly Imprinted Polymer Sensor for the Selective Recognition of Type II Collagen in Joint Degeneration Monitoring
    (2026-02-01) ;
    Vongmanee, Naphatsawan
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    Type II collagen is a primary fibrillar component of articular cartilage, and its early degradation is a key biomarker of joint-degenerative disorders such as osteoarthritis, rheumatoid arthritis, gout, etc. Reliable detection at low concentrations remains challenging due to limited assay accessibility, complex analytical procedures, and nonspecific responses in multicomponent biological matrices. This research reports the development of a Molecularly Imprinted Polymer (MIP)–based electrochemical sensor engineered for the selective recognition of type II collagen. A series of monomer formulations were evaluated, and the 1AAM:2VP composition produced a well-defined imprinted layer on screen-printed carbon electrodes, yielding the highest electrochemical sensitivity and linearity. The optimized sensor exhibited strong anodic and cathodic responses proportional to increasing collagen concentrations, with a calibration slope corresponding to an R<sup>2</sup> value of 0.9394. Minimal signal interference was observed, confirming high molecular selectivity. The limit of detection (LOD) was calculated to be approximately 0.065 µg/mL. These characteristics demonstrate that the proposed MIP sensor provides a low-cost, accessible, and highly selective analytical platform suitable for early-stage cartilage degeneration monitoring.
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    Biosensors for Klebsiella pneumoniae with Molecularly Imprinted Polymer (MIP) Technique
    (2022-06-01) ;
    Vongmanee, Naphatsawan
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    Sukjee, Wannisa
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    Sangma, Chak
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    Nosocomial infection is one of the most important problems that occurs in hospitals, as it directly affects susceptible patients or patients with immune deficiency. Klebsiella pneumoniae (K. pneu-moniae) is the most common cause of nosocomial infections in hospitals. K. pneumoniae can cause various diseases such as pneumonia, urinary tract infections, septicemias, and soft tissue infections, and it has also become highly resistant to antibiotics. The principal routes for the transmission of K. pneumoniae are via the gastrointestinal tract and the hands of hospital personnel via healthcare workers, patients, hospital equipment, and interventional procedures. These bacteria can spread rapidly in the hospital environment and tend to cause nosocomial outbreaks. In this research, we developed a MIP-based electrochemical biosensor to detect K. pneumoniae. Quantitative detection was performed using an electrochemical technique to measure the changes in electrical signals in different concentrations of K. pneumoniae ranging from 10 to 10<sup>5</sup> CFU/mL. Our MIP-based K. pneumoniae sensor was found to achieve a high linear response, with an R<sup>2</sup> value of 0.9919. A sensitivity test was also performed on bacteria with a similar structure to that of K. pneumoniae. The sensitivity results show that the MIP-based K. pneumoniae biosensor with a gold electrode was the most sensitive, with a 7.51 (% relative current/log concentration) when compared with the MIP sensor applied with Pseudomonas aeruginosa and Enterococcus faecalis, where the sensitivity was 2.634 and 2.226, respectively. Our sensor was also able to achieve a limit of detection (LOD) of 0.012 CFU/mL and limit of quantitation (LOQ) of 1.61 CFU/mL.
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    Study of ECG variation in daily activity
    (2017-12-19)
    Samona, Yanisa
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    Electrocardiogram (ECG) records electrical activity of the heart spreading through the heart muscle to make the heart contract. Recently ECG has been captured attention as biometric feature due to its uniqueness and large reliabilities for human identifications. In this study we aimed to verify the conservative ECG of human in their activities to ensure whether it is suitable to be used as biometric devices. Experiment studies involved 6 participants of which the age ranges is between 21 and 23. We test the robustness of ECG under various situation including health condition, emotional state and heart rate variation. The recorded ECG signal is forwarded for analysis using Matlab. Correlation coefficient of ECG Fourier transform is used as criterion to validate the ECG robustness. The result indicates that ECG is not stable and seems to vary with daily activity and emotional state. This will hampers ECG to be used as Biometric.