Rahong, Sakon
Loading...
Preferred name
Rahong, Sakon
Alternative Name
Rahong, S.
Main Affiliation
Email
sakon.ra@kmitl.ac.th
19 results
Now showing 1 - 10 of 19
- Some of the metrics are blocked by yourconsent settings
Item type:Publication, ZnO Nanorods Grown on Heterogenous Ag Seed Layers for Single-Cell Fluorescence Bioassays(2021-07-23) ;Muensri, Phitchaya ;Treetong, Alongkot ;Namdee, Katawut ;Kasamechonchung, PanitaWutikhun, TuksadonHere we demonstrate the controllability of the morphology of hydrothermal ZnO nanorods (ZnO-NRs) grown on heterogenous Ag seed layers. By varying the crystal orientation of silver thin films (Ag), a high density of ZnO-NRs could be obtained. We find that the density of ZnO-NRs strongly relates to the peak intensity ratio between (111) and (200) planes of Ag thin films due to a heteroepitaxy between (0002) ZnO and (111) Ag rather than that of grain boundary nucleation and/or surface nucleation. In addition, the optimized heterostructure of ZnO nanorod/Ag arrays is investigated via a critical concentration for nucleation and used as a fluorescence enhancement substrate (FES). The experimental results have shown that the FES presents an ability to detect a biological sample (PC-3 cell) with a high sensitivity and low detection limit of 1 cell/μL. Our results highlight that understanding an important key to control and design the morphology of heterogeneous hydrothermal ZnO-NR growth is essential to open up the opportunities for fundamental studies and applications in high-performance integrated nanodevices. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Metal Oxide Nanostructures Enhanced Microfluidic Platform for Efficient and Sensitive Immunofluorescence Detection of Dengue Virus(2023-11-01) ;Pormrungruang, Pareesa ;Phanthanawiboon, Supranee ;Jessadaluk, Sukittaya ;Larpthavee, PreedaThaosing, JiraphonRapid and sensitive detection of Dengue virus remains a critical challenge in global public health. This study presents the development and evaluation of a Zinc Oxide nanorod (ZnO NR)-surface-integrated microfluidic platform for the early detection of Dengue virus. Utilizing a seed-assisted hydrothermal synthesis method, high-purity ZnO NRs were synthesized, characterized by their hexagonal wurtzite structure and a high surface-to-volume ratio, offering abundant binding sites for bioconjugation. Further, a comparative analysis demonstrated that the ZnO NR substrate outperformed traditional bare glass substrates in functionalization efficiency with 4G2 monoclonal antibody (mAb). Subsequent optimization of the functionalization process identified 4% (3-Glycidyloxypropyl)trimethoxysilane (GPTMS) as the most effective surface modifier. The integration of this substrate within a herringbone-structured microfluidic platform resulted in a robust device for immunofluorescence detection of DENV-3. The limit of detection (LOD) for DENV-3 was observed to be as low as 3.1 × 10<sup>−4</sup> ng/mL, highlighting the remarkable sensitivity of the ZnO NR-integrated microfluidic device. This study emphasizes the potential of ZnO NRs and the developed microfluidic platform for the early detection of DENV-3, with possible expansion to other biological targets, hence paving the way for enhanced public health responses and improved disease management strategies. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, The enhancement of sensitivity and response times of PDMS-based capacitive force sensor by means of active layer modification(2021-06-01) ;Siangkhio, Yasumin; ; ; Jessadaluk, SukittiyaIn this work, sensitivity and response times of PDMS-based capacitive force sensors are enhanced via the modifications of the PDMS layer. Two modifying approaches are proposed; (i) change PDMS's (elastomer:curing agent) ratio and (ii) adding conductive polymer PEDOT:PSS into the PDMS layer. The change of PDMS (elastomer:curing agent) ratio from (10:1) to (30:1) increases the sensitivity from 0.4 0.08 to 0.72 0.23 kPa-1 (+80%) but it does not significantly affect the response/recovery times. In addition, by adding 1% wt. of PEDOT:PSS to PDMS (30:1), the further increment of sensitivity from 0.72 0.23 to 1.44 0.17 kPa-1 (+100%) and the shorter response time from 1.59 0.02 to 0.45 0.03 s (-72%) are observed. The mechanical and electrical studies reveal that the change of PDMS (elastomer:curing agent) ratio and the adding of PEDOT:PSS to PDMS layer result in the modification of PDMS's deformability and the increase of charge transportation, leading to the enhancement of sensing characteristics of the sensors. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Annealed zno/al2o3 core-shell nanowire as a platform to capture rna in blood plasma(2021-07-01) ;Takahashi, Hiromi ;Yasui, Takao ;Klamchuen, Annop; Wuthikhun, TuksadonRNA analytical platforms gained extensive attention recently for RNA-based molecular analysis. However, the major challenge for analyzing RNAs is their low concentration in blood plasma samples, hindering the use of RNAs for diagnostics. Platforms that can enrich RNAs are essential to enhance molecular detection. Here, we developed the annealed ZnO/Al<inf>2</inf>O<inf>3</inf> core-shell nanowire device as a platform to capture RNAs. We showed that the annealed ZnO/Al<inf>2</inf>O<inf>3</inf> core-shell nanowire could capture RNAs with high efficiency compared to that of other circulating nucleic acids, including genomic DNA (gDNA) and cell-free DNA (cfDNA). Moreover, the nanowire was considered to be biocompatible with blood plasma samples due to the crystalline structure of the Al<inf>2</inf>O<inf>3</inf> shell which serves as a protective layer to prevent nanowire degradation. Our developed device has the potential to be a platform for RNA-based extraction and detection. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Influence of Dragon Fruit Peels on the Synthesis of Antibacterial Nano Zinc Oxide (Nano-ZnO) via Green Synthesis Method(2026-05-20) ;Sakulpeeb, Natchayaporn ;Koetniyom, Wantana; ; This research focused on adding value to dragon fruit peel waste by utilizing it in the synthesis of antibacterial nano zinc oxide (Nano-ZnO) through a green synthesis process. In this study, all the dragon fruit peels were extracted using the solvent extraction technique with three different solvents (deionized water, ethanol, and methanol) for 1, 2, 3, 4, and 5 h, respectively. The amount of flavonoids from the extract was determined using UV-Vis spectrophotometer to obtain the optimum extraction time, which was 4 h for DI water as the solvent. Moreover, antibacterial Nano-ZnO was synthesized successfully by a green synthesis process using zinc nitrate Zn(NO3)2 and the extracts. The molecular vibrations as well as the crystal structure and morphology were investigated by Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy (Raman), X-ray diffraction (XRD), and field emission scanning electron microscopy (FE-SEM), respectively. Additionally, the antibacterial efficacy of the nano-zinc oxide samples was evaluated using disc diffusion method. Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) were the test agents. The research shows that the X-ray diffraction patterns of all synthesized ZnO nanoparticles (NPs) exhibited a wurtzite (hexagonal) crystal structure. FT-IR spectroscopy confirmed the presence of Zn-O stretching vibrations at approximately 500 cm⁻¹. Furthermore, the FE-SEM reveals that ZnO-yellow particles displayed spherical morphologies with an average particle size of 145 nm. At the same time, ZnO-White and ZnO-Red nanoparticles exhibited a combination of rod-like and elliptical morphologies, with average particle sizes of 168 nm and 321 nm, respectively. In addition, the antibacterial activity demonstrates effective inhibition against S. aureus and E. coli in all three ZnO nanoparticle conditions. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Mutation detection of urinary cell-free DNA via catch-and-release isolation on nanowires for liquid biopsy(2023-08-15) ;Takahashi, Hiromi ;Yasui, Takao ;Hirano, Masaki ;Shinjo, KeikoMiyazaki, YusukeCell-free DNA (cfDNA) and extracellular vesicles (EVs) are molecular biomarkers in liquid biopsies that can be applied for cancer detection, which are known to carry information on the necessary conditions for oncogenesis and cancer cell-specific activities after oncogenesis, respectively. Analyses for both cfDNA and EVs from the same body fluid can provide insights into screening and identifying the molecular subtypes of cancer; however, a major bottleneck is the lack of efficient and standardized techniques for the isolation of cfDNA and EVs from clinical specimens. Here, we achieved catch-and-release isolation by hydrogen bond-mediated binding of cfDNA in urine to zinc oxide (ZnO) nanowires, which also capture EVs by surface charge, and subsequently we identified genetic mutations in urinary cfDNA. The binding strength of hydrogen bonds between single-crystal ZnO nanowires and DNA was found to be equal to or larger than that of conventional hydrophobic interactions, suggesting the possibility of isolating trace amounts of cfDNA. Our results demonstrated that nanowire-based cancer screening assay can screen cancer and can identify the molecular subtypes of cancer in urine from brain tumor patients through EV analysis and cfDNA mutation analysis. We anticipate our method to be a starting point for more sophisticated diagnostic models of cancer screening and identification. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Selective formations of antimony-dopant for highly sensitive nitrogen dioxide responsive behavior of tin oxide-based chemiresistive sensor(2025-02-15) ;Rattanawarinchai, Prapakorn; ; ; Here, selective formation of antimony (Sb) dopant species responsible for highly sensitive gas sensors based on tin oxide (SnO<inf>2</inf>) film grown via pulsed laser deposition is presented. By elevating a forming energy through controlling substrate temperature, not only crystallinity of Sb-SnO<inf>2</inf> (ATO) is notably enhanced but the Sb<sup>5 +</sup> also predominantly replace at Sn<sup>4+</sup> site rather than Sb<sup>3+</sup> counterpart. Such Sb-species selection plays a crucial role on the density of oxygen vacancy and free electron enabling to rationally design conductive behaviour of ATO film from insulative to degenerated semiconductor. As a practical example, detection of nitrogen dioxide (NO<inf>2</inf>) gas is selected as an application model. We found a narrow window for high NO<inf>2</inf> sensing performance of ATO film which strongly corresponds with the amount of carrier density. At certain window, ATO film exhibits high NO<inf>2</inf> response of 24.65 (10 ppm) and low limit of detection of 0.5 ppm, which is 5-fold higher and 10-fold lower than that of undoped-SnO<inf>2</inf>, respectively. Our finding demonstrates a facile approach to design over the chemical state, defect, and conductivity of the active sensing layer, allowing us to achieve an excellent sensing performance of functional materials conjugated to a nano-electronic platform. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Influence of Antimony Species on Electrical Properties of Sb-Doped Zinc Oxide Thin Films Prepared by Pulsed Laser Deposition(2023-06-01) ;Jessadaluk, Sukittaya; ; ; This study systematically investigates the influence of antimony (Sb) species on the electrical properties of Sb-doped zinc oxide (SZO) thin films prepared by pulsed laser deposition in an oxygen-rich environment. The Sb species-related defects were controlled through a qualitative change in energy per atom by increasing the Sb content in the Sb<inf>2</inf>O<inf>3</inf>:ZnO-ablating target. By increasing the content of Sb<inf>2</inf>O<inf>3</inf> (wt.%) in the target, Sb<sup>3+</sup> became the dominant Sb ablation species in the plasma plume. Consequently, n-type conductivity was converted to p-type conductivity in the SZO thin films prepared using the ablating target containing 2 wt.% Sb<inf>2</inf>O<inf>3</inf>. The substituted Sb species in the Zn site (Sb<inf>Zn</inf><sup>3+</sup> and Sb<inf>Zn</inf><sup>+</sup>) were responsible for forming n-type conductivity at low-level Sb doping. On the other hand, the Sb–Zn complex defects (Sb<inf>Zn</inf>–2V<inf>Zn</inf>) contributed to the formation of p-type conductivity at high-level doping. The increase in Sb<inf>2</inf>O<inf>3</inf> content in the ablating target, leading to a qualitative change in energy per Sb ion, offers a new pathway to achieve high-performing optoelectronics using ZnO-based p–n junctions. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Electroreflectance study of antimony doped ZnO thin films grown by pulsed laser deposition(2021-10-01) ;Jessadaluk, Sukittaya; ;Rattanawarinchai, Prapakorn; In this research, antimony doped ZnO (SZO) thin films with various doping content have been grown on a c-Al<inf>2</inf>O<inf>3</inf> substrate by pulsed laser deposition. The effect of the applied electric field on the bandgap of SZO thin films was studied by electroreflectance (ER) spectroscopy using a capacitor-type geometry. Hall effect measurements indicate that the p-type conductivity of SZO is realized for the Sb<inf>2</inf>O<inf>3</inf> weight percentage at 2%. The blue shift of the energy bandgap was observed in thin films after increasing the doping concentration. The Burstein-Moss effect is the crucial mechanism for the blue shift of the SZO bandgap. Furthermore, we found the red shift of bandgap in all samples, which was measured under various electric fields by ER spectroscopy. The changes of the optical transition in the band structure should be the origin of the red shift behaviors of the SZO bandgap under the presence of the electric field. Based on our results, we can design and optimize the bandgap of SZO for optoelectronic devices. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Enhancement of sensing characteristics of Polydimethylsiloxane-based capacitive force sensor by introducing conductive polymer to dielectric layer(2021-01-01) ;Siangkhio, Yasumin; ; ; Jessadaluk, SukittayaA capacitive force sensor is one of the electronics components used in several electronic devices and applications. An improvement of sensing characteristics of the sensor, for example sensitivity and response time, becomes an interesting research topic. The alternative approach to enhance the sensitivity and response time of polydimethylsiloxane-based capacitive force sensors is proposed by introducing poly(3,4-ethylenedioxythiophene) polystyrene sulphonate, a conductive polymer, into polydimethylsiloxane active layer. Two sensors using different active layers, (i) polydimethylsiloxane (conventional sensor) and (ii) poly(3,4-ethylenedioxythiophene) polystyrene sulphonate mixed polydimethylsiloxane (modified sensor), were fabricated and characterised to reveal the sensing enhancement. Interestingly, the modified sensor shows the significant increase in the sensitivity from 0.7 to 1.14 kPa<sup>–1</sup> (+62.86%) and the shortening response time from 1.55 to 0.43 s (−72.26%) with respect to the conventional sensor. In addition, the deterioration in elastic behaviour and the faster charge–discharge behaviour observed from the poly(3,4-ethylenedioxythiophene) polystyrene sulphonate mixed polydimethylsiloxane film indicate the better deformation and charge transport than that from polydimethylsiloxane film. Therefore, it can be concluded that the conductive poly(3,4-ethylenedioxythiophene) polystyrene sulfonate additive plays the role of mechanical and electrical modification of the polydimethylsiloxane active layer leading to the enhancement in sensitivity and response time of the polydimethylsiloxane-based capacitive force sensor.
